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無內(nèi)毒素質(zhì)粒 DNA Midi 試劑盒 *

簡要描述:NucleoBond Xtra Midi EF,用于無內(nèi)毒素質(zhì)粒 DNA 的 Midi 試劑盒
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無內(nèi)毒素質(zhì)粒 DNA Midi 試劑盒 *
NucleoBond Xtra Midi EF, Midi kit for endotoxin-free plasmid DNA

  • 產(chǎn)品型號:MN 740420.5
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2021-10-22
  • 訪  問  量:2406

詳細介紹

品牌其他品牌貨號MN 740420.5
供貨周期現(xiàn)貨應(yīng)用領(lǐng)域醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥,綜合

無內(nèi)毒素質(zhì)粒 DNA  Midi 試劑盒 *

無內(nèi)毒素質(zhì)粒 DNA  Midi 試劑盒 *

NucleoBond Xtra Midi EF, Midi kit for endotoxin-free plasmid DNA

NucleoBond Xtra Midi EF,用于無內(nèi)毒素質(zhì)粒 DNA 的 Midi 試劑盒

ApplicationEndotoxin-free plasmid DNA isolation
TargetPlasmid DNA
CE certifiedNo, research use only
TechnologyAnion exchange chromatography
BrandNucleoBond
FormatMidi prep
Package unit10 Prep(s), 50 Prep(s)
HandlingGravity flow
Lysate clarificationColumn filter
Automated useYes, can be automated on the Andrew+ the pipetting robot
Sample materialBacteria, E. coli
Sample amount< 200 mL (high copy), < 400 mL (low copy)
Vector size< 300 kbp
Typical yield500 µg
Theoretical binding capacity800 µg
Typical purity A260/A2801.8?1.95
Endotoxin level< 0.1 EU/µg DNA
Isopropanol precipitationCentrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi
Preparation time85 min/prep
Typical downstream applicationCloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature15?25 °C
Shelf life (from production)39 Months


品牌貨號品名規(guī)格
MACHEREY-NAGEL(MN)740413NucleoBond Smart Rack(試劑)PK
MACHEREY-NAGEL(MN)740427NucleoBond Xtra EF Buffer Set I(試劑)PK
MACHEREY-NAGEL(MN)740579NucleoBond BAC 100 (10)(試劑)PK
MACHEREY-NAGEL(MN)740300.5NucleoSnap DNA Plasma (50 preps)(試劑)PK
MACHEREY-NAGEL(MN)740329.1Buffer LYS (1000 mL)(試劑)PK
MACHEREY-NAGEL(MN)740348.1Buffer NEU (1000 mL)(試劑)PK
MACHEREY-NAGEL(MN)740363.1Buffer RES (1000 mL)(試劑)PK
MACHEREY-NAGEL(MN)740386.1Buffer RES-EF (1000 mL)(試劑)1PAC
MACHEREY-NAGEL(MN)740387.1Buffer LYS-EF (1000 mL)(試劑)1PAC
MACHEREY-NAGEL(MN)740388.1Buffer NEU-EF(試劑)1000 mL
MACHEREY-NAGEL(MN)740393.9Buffer ELU-EF(試劑)900 mL
MACHEREY-NAGEL(MN)740404.2NucleoZOL (200 mL)(試劑)PK
MACHEREY-NAGEL(MN)740406.5NucleoSpin RNA Set for NucleoZOL(試劑)PK
MACHEREY-NAGEL(MN)740410.1NucleoBond Xtra Midi (10)(試劑)PK
MACHEREY-NAGEL(MN)740410.5NucleoBond Xtra Midi (50)(試劑)PK
MACHEREY-NAGEL(MN)740412.1NucleoBond Xtra Midi Plus (10)(試劑)PK
MACHEREY-NAGEL(MN)740412.5NucleoBond Xtra Midi Plus (50)(試劑)PK
MACHEREY-NAGEL(MN)740414.1NucleoBond Xtra Maxi (100)(試劑)PK
MACHEREY-NAGEL(MN)740414.5NucleoBond Xtra Maxi (50)(試劑)PK
MACHEREY-NAGEL(MN)740416.1NucleoBond Xtra Maxi Plus (10)(試劑)PK
MACHEREY-NAGEL(MN)740416.5NucleoBond Xtra Maxi Plus (50)(試劑)PK
MACHEREY-NAGEL(MN)740420.1NucleoBond Xtra Midi EF (10)(試劑)PK
MACHEREY-NAGEL(MN)740420.5NucleoBond Xtra Midi EF (50)(試劑)PK
MACHEREY-NAGEL(MN)740422.1NucleoBond Xtra Midi Plus EF (10)(試劑)PK
MACHEREY-NAGEL(MN)740422.5NucleoBond Xtra Midi Plus EF (50)(試劑)PK
MACHEREY-NAGEL(MN)740424.5NucleoBond Xtra Maxi EF (50)(試劑)PK
MACHEREY-NAGEL(MN)740426.1NucleoBond Xtra Maxi Plus EF (10)(試劑)PK
MACHEREY-NAGEL(MN)740426.5NucleoBond Xtra Maxi Plus EF (50)(試劑)PK
MACHEREY-NAGEL(MN)740436.1NucleoBond Xtra BAC (10)(試劑)PK
MACHEREY-NAGEL(MN)740490.25NucleoSpin Plasmid Transfection-grade(試劑)250preps
MACHEREY-NAGEL(MN)740609.25NucleoSpin Gel and PCR Clean-up (250)(試劑)PK
MACHEREY-NAGEL(MN)740609.5NucleoSpin Gel and PCR Clean-up (50)(試劑)PK
MACHEREY-NAGEL(MN)740691.4NucleoSpin 96 Virus (4x96)(試劑)PK
MACHEREY-NAGEL(MN)740787.4NucleoSpin 96 Soil (4x96)(試劑)PK
MACHEREY-NAGEL(MN)740945.25NucleoSpin Food (250)(試劑)PK
MACHEREY-NAGEL(MN)740945.5NucleoSpin Food (50)(試劑)PK
MACHEREY-NAGEL(MN)740952.5NucleoSpin Tissue (50)(試劑)PK
MACHEREY-NAGEL(MN)740955.5NucleoSpin RNA (50)(試劑)PAC
MACHEREY-NAGEL(MN)745120.5Protino Ni-TED 2000 packed columns (5)(試劑)PK

第二代陰離子交換劑,可節(jié)省無內(nèi)毒素質(zhì)粒 DNA

應(yīng)用無內(nèi)毒素質(zhì)粒 DNA 分離
目標質(zhì)粒DNA
CE認證不,僅供研究使用
技術(shù)陰離子交換色譜
核鍵
格式迷笛準備
包裝單元10 次準備,50 次準備
處理重力流
裂解液澄清列過濾器
自動化使用是的,可以在 Andrew+ 移液機器人上實現(xiàn)自動化
樣品材料細菌,大腸桿菌
樣品量< 200 mL(高拷貝),< 400 mL(低拷貝)
矢量大小< 300 kbp
典型產(chǎn)量500 微克
理論結(jié)合力800 微克
典型純度 A260/A2801.8-1.95
內(nèi)毒素水平< 0.1 EU/µg DNA
異丙醇沉淀離心、NucleoBond 終結(jié)器、NucleoSnap Finisher Midi、NucleoSpin Finisher Midi
準備時間85 分鐘/準備
典型下游應(yīng)用克隆、PCR、限制性分析、測序、細胞轉(zhuǎn)染
儲存溫度15-25℃
保質(zhì)期(從生產(chǎn)開始)39個月

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